Биоинформатика. Порозов Ю.Б. - 49 стр.

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We have now identified a novel cis-acting element within the HIV-2

enhancer, immediately upstream of the kappa B site , designated peri-kappa B.

This site is conserved among isolates of HIV-2 and the closely related simian

immunodeficiency virus , and transfection assays show this site to mediate HIV-

2 enhancer activation following stimulation of monocytic but not T-cell lines .

This is the first description of an HIV-2 enhancer elementwhich displays such

monocyte specificity, and no comparable enhancer element has been clearly

defined for HIV-1.

While a nuclear factor( s ) from both peripheral blood monocytes and T cells

binds the peri-kappa B site, electrophoretic mobility shift assays suggest that

either a different protein binds to this site in monocytes versus T cells or that the

protein recognizing this enhancer element undergoes differential modification in

monocytes and T cells, thus supporting the transfection data.

Further , while specific constitutive binding to the peri-kappa B site is seen in

monocytes, stimulation with phorbol esters induces additional, specific binding.

Understanding the monocyte-specific function of the peri-kappa B factor may

ultimately provide insight into the different role monocytes and T cells play in

HIV pathogenesis.

Лабораторная работа № 8

Цель работы

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изучение возможностей горизонтального переноса

генов у бактерий на примере т-РНК синтетазы (рисунок 4).

Рисунок 4. Работа т-РНК синтетазы.

Роль т-РНК синтетазы.

При помощи BLAST выберите последовательности бактериальных

т-РНК. Постройте филогенетическое дерево, используя выбранные

последовательности.